Contipro A.s.

Contipro Biotech S.r.o.

Miroslava Nemcova

Vladimir Velebny

Kristina Nesporova

Gloria Huerta-Angeles

Lucie Wolfova

Jaromir Kulhanek

Jaroslav Novotny

Martina Hermannova

Daniela Smejkalova

Klara Slezingrova

Martin Pravda

Lucie Vistejnova

Krzysztof Niedoba

Eva Prikopova

Pavel Klein

Marcela Foglarova

Dagmar Cozikova

Jana Sogorkova

Martin Bobek

Jan Kucera

Klara Slezingerova

Krzystof Niedoba

The invention relates to a method of preparation hydrophobized hyaluronic acid (Formula I) and further to a method of encapsulating biologically active substances into the nanomicelles of hydrophobized hyaluronan serving as carriers of biologically active hydrophobic substances. The hydrophobization of hyaluronan is carried out through an esterification reaction of hyaluronan with long-chain carboxylic acids, the latter being activated by a halogenide derivative of 2,4,6-trichlorobenzoic acid or by another organic chloride. In an aqueous environment, water-soluble hydrophobized derivatives can form nanomicelles in which nonpolar substances can be bound by means of non-covalent physical interactions. The core of a nanomicelle is formed by hydrophobic acyl functional groups while the shell of a nanomicelle is formed by hyaluronic acid. The encapsulation of the substances into nanomicelles can be performed by means of the solvent exchange method or by means of sonication. Hyaluronic nanomicelles support the penetration of bound substances in topical applications and enable the bound substances to be transferred into the individual cells. The nanomicelles obtained from hydrophobized hyaluronan derivatives are usable in cosmetic and pharmaceutical applications. 

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The invention related to hyaluronic derivative according to formula (I), methods of preparation thereof and a hydrogel prepared obtained from the derivative and methods of preparation thereof. The hydrogel can be used in tissue engineering, cosmetics, medicine or regenerative medicine such as the forming of scaffolds for the treatment of articular cartilage or bone tissue defects. 

Derivatives of hyaluronic acid capable of forming hydrogels

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Chocen, Czechia

Miroslava Nemcova