Ceskoslovenska Akademie Ved

Miroslava Krivakova

Jiri Coupek

Jiri Hradil

Svatopluk Pokorny

Drahoslav Lim

Otto Wichterle

Jiri Stamberg

Jaroslava Turkova

Otakar Mikes

Petr Strop

Jaromir Lukas

Viktor Mansfeld

Arthur Stoy

Jaroslava Trukova

Olga Hubalkova

This invention relates to a method for preparing globular, hydrophilic copolymers which have reticulated internal structures and are suitable for gas and gel chromatography. The method comprises polymerization by suspension polymerization methods of hydrophilic and hydrophilic monomers in aqueous medium in the presence of a component which prevents transfer of the hydrophilic monomer into the aqueous medium and the solubility of the aqueous medium in the monomeric phase.

Preparation of hydrophilic material for gel chromatography

The invention relates to preparation of synthetic macroporous ion-exchanaging materials by a ternary copolymerization of hydrophilic monomers containing non-ionogenous groups with monomers containing ionogenous groups and cross-linking monomers in an aqueous dispersion in the presence of inert components and suspension stabilizers. Hydroxyalkyl, oligoglycol and polyglycol esters and N-substituted or unsubstituted amides of acrylic and methacrylic acid are used as the hydrophilic monomers; compounds CH.sub.2 .dbd.C(R)COX, where R = H or CH.sub.3, X = --OR.sub.1 N(R.sub.2)R.sub.3, --OR.sub.1 SO.sub.3 H, --NHR.sub.1 N(R.sub.2)R.sub.3 or --HNR.sub.1 SO.sub.3 H, R.sub.1 = alkylene and R.sub.2 and R.sub.3 = H, alkyl, hydroxyalkyl or aminoalkyl, acrylic and methacrylic acid serve as the ionogenous monomers; the crosslinking agents are selected from a group of monomers consisting of alkylene, oligoglycol or polyglycol diacrylates and dimethacrylates, alkylenebisacrylamides, alkylenebismethacrylamides, divinylbenzene and other compounds containing more than 2 polymerizable acryloyl or methacryloyl groups. The invented materials are suitable above all for isolation and chromatographic separation of sensitive compounds of the biological origin.

Method for preparation of hydrophilic polymeric ion exchanging gels

The invention relates to macroporous polymeric sorbents prepared by ternary copolymerization of polar monomers, namely hydroxyalkyl acrylates and methacrylates (hydroxyalkyl C.sub.1-6), in the amount of 1 - 70 wt.%, with 30 - 90 wt.% of nonpolar comonomers, namely alkyl acrylates and methacrylates (alkyl C.sub.1-18), and 20 - 80 wt.% of crosslinking agents, namely alkylene diacrylates and dimethacrylates (alkylene C.sub.1-6). The sorbents were prepared by the suspension copolymerization in the presence of inert organic solvents and surfactants in the single step without further chemical modification and their polarity was controlled in a broad region. They are especially suitable for gas-chromatographic separation of organic compounds, as hydrocarbons, alcohols, organic acids and nitrogen-containing heterocyclic compounds. Their preparation in one step is more simple and economical than modification of the polymeric matrix by polymeranalogous reactions which has been used for this purpose until present time.

Macroporous polymeric sorbents for chromatography, especially gas

There is disclosed a composition of matter comprising a polymer containing aromatically bound mercuric ions having the formula ##STR1## wherein R is H or CH.sub.3, Y is -- CO(OCH.sub.2.CH.sub.2).sub.x -- O --, wherein x = 0-20, or -- CONH --, Ar is a bifunctional aromatic group, Z = H or NH--R.sup.2 wherein R.sup.2 is acyl, carboxy C -- to -- alkyl or a sulfonyl group, and R.sup.1 is the chain of homopolymer of the formula ##STR2## or the chain of a copolymer selected from the group consisting of methacrylic or acrylic esters of polyfunctional alcohols, methacrylamides or acrylamides, monomers containing one or more acrylic or methacrylic acid groups and monomers of the acrylic or methacrylic series, method of preparing such polymers, carriers produced on the basis of such polymers and method of extracting sulfur-containing compounds from mixtures containing the same utilizing the polymers of the invention.

Mercurated polymers, method for their preparation and polymers produced

A method for the isolation of biologically active compounds by affinity chromatography comprising forming a sorption complex between a solvent soluble biologically active compound to be isolated and biologically active compound linked to a solid carrier by a covalent bond, said solid carrier being a hydrophillic macroporous copolymer.

Isolation of biologically active compounds by affinity chromatography

Biologically active compounds such as enzymes are insolubilized by hydrophilizing a macroporous substantially hydrophobic carrier by hydrolysis of a thin surface layer of the carrier to form free carboxylic acid groups, and thereafter activating the carrier and reacting the activated carrier with a biologically active compound.

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Prague, Czechia

Miroslava Krivakova