Harvard College

Columbia University

Roche Sequencing Solutions, Inc.

Mirkó Palla

George M Church

Jingyue Ju

Shiv R Kumar

James J Russo

Aruna Ayer

Jeffrey Matthew Nivala

Peter Benjamin Stranges

Francois Pepin

Sukanya Srinivasa Rao Arun Punthambaker

Peter B Stranges

The present disclosure is directed to compositions and methods for deriving a plurality of kinetics parameters () for at least two different enzyme variants in a multiplex manner using nanopore-based sequencing. In some embodiments, the systems and methods may be used to screen different nanopore variants, or different combinations of both nanopore variants and enzyme variants.

Enzyme screening methods

This invention provides methods and systems of DNA synthesis including providing an encoding unit comprising an enzyme, a single-stranded DNA (ssDNA) and a nanopore, providing a lipid bilayer having on opposite sides a cis and a trans reservoir each having a different buffer composition, wherein the nanopore is within the lipid bilayer and the enzyme and the ssDNA are in the cis reservoir, providing an electrode over the lipid bilayer wherein the electrode can modulate voltage across the lipid bilayer, wherein the enzyme catalyzes DNA synthesis in response to the voltage.

Method and system of nanopore-based information encoding

This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties.

Raman cluster tagged molecules for biological imaging

Growing community of inventors

Newton, MA, United States of America

Mirkó Palla