Evonik Degussa Gmbh

Evonik Operations Gmbh

Technische Universitat Munchen

Mirja Wessel

Steffen Schaffer

Anja Thiessenhusen

Thomas Haas

Hans-Georg Joerg Hennemann

Harald Haeger

Arne Skerra

Hans Henning Wenk

Oliver Thum

Jan Christoph Pfeffer

Martin Schilling

Christoph Schorsch

Nadine Stein

Jasmin Corthals

Anne Jeremias

Mike Farwick

Martin Roos

Klaus-Dieter Vorlop

Kathrin Daniela Brandt

Markus Poetter

Wolfgang Kroutil

Florian Klasovsky

Harald Häger

Jasmin Gielen

Frank Erhardt

Heiko Andrea

Markus Pötter

Christian Gehring

Wilfried Blümke

Maciej Olek

Yvonne Schiemann

Philip Engel

Stefan Julian Liebig

Michael Volland

Eva-Maria Eckl

Sabine Hafkemeyer

Wilfried Bluemke

Michaela Hauberg

Anja Hecker

Katharina Berse

Marion Schiebelhut

Ludwig Kirmair

Silvana Roeder

Thomas Willke

Rene Schöbel

Markus PÖTTER

Harald Hager

Hendrik Krauter

Thomas Tacke

The invention relates to a mixture composition comprising glucolipids, to its use for producing formulations and to formulations comprising this mixture composition.

Mixture composition comprising glucolipids

The invention relates to cells which make rhamnolipids and are genetically modified such that they have a decreased activity, compared to the wild type thereof, of a glucose dehydrogenase and to a method for producing rhamnolipids using the cells according to the invention.

Rhamnolipid-producing cell having reduced glucose dehydrogenase activity

The present invention relates to at least one cell for producing at least one lipid with general formula II from at least one carbon substrate, wherein Rand Rindependently of one another comprises identical or different organic radicals each with 5 to 13 carbon atoms,wherein the cell is a non-pathogenic cell that is genetically modified to increase the heterologous expression relative to the wild type cell of:

Lipid production

There is provided a microbial cell expressing a mutant AlkB enzyme, the mutant AlkB enzyme comprising at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position V129 and/or T136 of the wild type AlkB enzyme. There is also provided a method for producing omega-hydroxy carboxylic acid and/or ester thereof using this cell.

Microorganism expressing mutant AlkB enzyme and use to prepare omega-hydroxy carboxylic acid and/or ester

The invention provides a process for reacting a carboxylic acid ester of the formula (I)R-A-COOR&#x2003;&#x2003;(I),

Process for reacting a carboxylic acid ester

The present invention relates to a method of preparing at least one rhamnolipid comprising:

Methods of producing rhamnolipids

A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.

Method for aerobically producing alanine or a compound produced using alanine

The invention relates to a whole-cell catalyst which expresses a recombinant &#x3b1;-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the &#x3b1;-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an &#x3b1;-dioxygenase and contacting the product with a transaminase.

Producing amines and diamines from a carboxylic acid or dicarboxylic acid or a monoester thereof

The invention provides a whole cell catalyst which expresses a recombinant &#x3b1;-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the &#x3b1;-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, &#x3c9;-hydroxy fatty acid, &#x3c9;-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a &#x3b1;-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

Production of omega-amino fatty acids

The invention relates to a method for producing a dermatologically active yeast extract, comprising the following steps: providing a preculture of the yeast cells, culturing the cells for at least fifteen minutes at a pH of 1.8-4, harvesting the cells and lysing the cells, and a yeast extract produced thereby and products comprising said yeast extract.

Dermatologically effective yeast extract

The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C &#x2014;(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.

Enzymatic omega-oxidation and omega-amination of fatty acids

This invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids.

Cells and methods for producing rhamnolipids

A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of &#x3b2;-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the &#x3b2;-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

Process for the improved separation of a hydrophobic organic solution from an aqueous culture medium

The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids.

Cells, nucleic acids, enzymes and use thereof, and methods for the production of sophorolipids

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Bochum, Germany

Mirja Wessel