Fundacao Oswaldo Cruz-Fiocruz

Fundacao Oswaldo Cruz

Other

Ouro Fino Agronegocio Ltda.

Alvos—consultoria, Desenvolvimento E Comercialização De Produtos Biotecnológicos S/a

Fundacaco Oswaldo Cruz-Fiocruz

Miriam Tendler

Naftale Katz

Andrew John George Simpson

Frank Jefferson Alarcon De Barrientos

Mônica Magno Vilar

Marilia Sirianni Dos Santos Almeida

Celso Raul Romero Ramos

Richard Charles Garratt

Andrew J G Simpson

Paulo Lee Ho

Andrew John Simpson

Marilia Sirianni Dos Santo Almeida

Isaias Raw

Richard Charles Garrat

Marilia Sirianni Almeida

Monica Magno Vilar

Frank Jefferson De Barrientos

Geraldo Rodrigues Garcia ARMÔA

Douglas Mcintosh

The present invention relates to peptide fragments which have one or more shared and/or similar amino acid sequences to amino acid sequences of specific portions of the 14 kDa protein of(SM14) or related FABPs (Fatty Acid Binding Proteins), the peptide fragments functioning as continuous or discontinuous epitopic regions of the molecule or mimicking its biological activity. More particularly, the present invention relates to a method for constructing active peptide fragments, peptide fragments, immunogenic compositions and diagnostic kits using peptide fragments.

Synthetic active peptide fragments

A process for purification of a fatty acid binding proteins such as, e.g., Sm14 ofor type-3 FABP protein of. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration.

Process for purification of a fatty acid binding protein

The production of recombinant proteins using a synthetic gene for increased expression of the protein inis disclosed. More specifically, disclosed is a process for producing a recombinant Sm14 protein of, by providing a synthetic gene for increased production of Sm14 protein, producing the gene and genetically manipulating thestrain with the gene in order to produce a vaccine effectively. Improved methods are also provided for producing and purifying this protein fromcells, which can be scaled for industrial production.

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The present invention relates to peptide fragments which have one or more shared and/or similar amino acid sequences to amino acid sequences of specific portions of the 14 kDa protein of(Sm14) or related FABPs (Fatty Acid Binding Proteins), the peptide fragments functioning as continuous or discontinuous epitopic regions of the molecule or mimicking its biological activity. More particularly, the present invention relates to a method for constructing active peptide fragments, peptide fragments, immunogenic composition and diagnostic kit using peptide fragments of the present invention.

Direct or indirect diagnostic test for helminth infection

The present invention relates to peptide fragments which have one or more shared and/or similar amino acid sequences to amino acid sequences of specific portions of the 14 kDa protein of(Sm14) or related FABPs (Fatty Acid Binding Proteins), the peptide fragments functioning as continuous or discontinuous epitopic regions of the molecule or mimicking its biological activity. More particularly, the present invention relates to a method for constructing active peptide fragments, peptide fragments, immunogenic composition and diagnostic kit using peptide fragments.

The primary objective of the present invention is the development of new mutant forms of the Sm14 protein, for producing a greater production volume. The recombinant proteins here obtained were capable of providing protection againstandinfection. The level of protection of Sm14 recombinant proteins obtained in the present invention was similar to that reached in the parasite saline extract. The mutant proteins of the present invention have reached approximately 100% of renaturation after the heating at 80&#xb0; C., different from wild forms of the Sm14 protein. Moreover, after storage for 2 months at 4&#xb0; C., mutant proteins have shown smaller &#x3b2;-structure loss than wild forms that have shown formation with random structure, as demonstrated by the circular dichroism analysis, indicating the success of mutations.

Helminth-derived antigens having capacity of providing protection against parasites

The present invention relates to helminthic derived antigenic material capable of inducing effective and long lasting protection against parasites, in particular to antigens that mediate protective immunity against helminths.

Vaccine against helminth infection comprising Sm-14 fatty acid binding

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Rio de Janeiro, Brazil

Miriam Tendler