Era Biotech, S.a.

Zera Intein Protein Solutions, S.l.

Miriam Bastida Virgili

Margarita Torrent Quetglas

Pablo Marzábal Luna

Blanca Llompart Royo

Pablo Marzabal Luna

Peter Bernard Heifetz

Maria Dolores Ludevid Múgica

Roser Pallisse Bergwerf

Ma Immaculada Ilop Tous

Kevin James O'Connor

Ma Dolores Ludevid Múgica

Minu Joseph

Maria Immaculada Llop Tous

Kevin James O Connor

M~ Inmaculada Llop

Joseph Minu

María Dolores Ludevid Mugica

Mª Inmaculada Llop

Polypeptide sequences for inducing recombinant protein bodies are described. The sequences comprise a polyproline II (PPII) structure and/or a proline-rich sequence between two cysteine residues on either end. Recombinant protein bodies are useful for protein production because they allow for simple and efficient purification of high quantities of recombinant protein. In addition, other methods of using recombinant protein bodies, for example, in vaccination and food products, are also described.

Recombinant protein body-inducing polypeptides

A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed. More particularly, a biologically active polypeptide fused to a protein sequence that mediates the induction of RPBLA formation is expressed and accumulated in host cells after transformation with an appropriate vector. The eukaryotic host cell does not produce protein bodies in the absence of the fusion protein. Methods for preparing and using the RPBLAs and the fusion protein are also disclosed, as are nucleic acid molecules that encode the fusion proteins.

Production of biologically active proteins

Growing community of inventors

Molins de Rei, Spain

Miriam Bastida Virgili