New England Biolabs, Inc.

Minyong Chen

James C Samuelson

Christopher H Taron

Ming-Qun Xu

Aihua Zhang

Margaret Heider

Xiaofeng Shi

Pingfang Liu

Ming-Oun Xu

Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4&#xb0; C.-40&#xb0; C. and is inactivated by raising the temperature to above 50&#xb0; C., where the proteinase is substantially inactive at 65&#xb0; C.

Proteinases with improved properties

Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.

Universal N-glycan binding reagent

Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.

Growing community of inventors

Ipswich, MA, United States of America

Minyong Chen