Nippon Kayaku Kabushiki Kaisha

Ikeda Food Research Co., Ltd.

Minoru Masuda

Masahiko Yabuuchi

Tsuneo Nakamura

Hiroshi Akanuma

Hiroshi Hayami

Masashi Hashiba

Kazuo Katoh

Shigeru Tajima

Hideki Yoshioka

Shuhei Tsukamoto

Masachika Hirayama

Tomoko Takezawa

Hironori Omura

Hirokazu Sanada

Emi Ishimaru

Reiko Machida

Yoshihiko Umegae

Mamoru Kunishima

Kazuo Kato

Kikuo Imazumi

Hiroaki Hamano

The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.

Method of determining 1,5-anhydroglucitol, and reagent composition for determining 1,5-anhydroglucitol

A protein comprising an amino acid sequence having at least one mutation selected from a Gly-4 to Ala mutation, a Glu-6 to His mutation, a Ser-14 to Thr mutation, an Ala-37 to Thr or Arg mutation, a Pro-50 to Gln mutation, a Glu-67 to Gly mutation, an Asp-80 to Tyr mutation, a Val-93 to Met mutation, an Arg-156 to Pro mutation, a Leu-164 to Met mutation, an Asn-202 to Asp mutation, a Thr-235 to Ala mutation, an Asn-348 to Tyr mutation, a Gly-362 to Arg mutation and a Val-473 to Ala mutation in the amino acid sequence depicted in SEQ II NO:4. (2) A thermostable protein which comprises an amino acid sequence derived from the amino acid sequence having at least one variation described in (1) and having 1,5-anhydroglucitol dehydrogenase activity. These proteins act specifically on 1,5-anhydroglucitol (1,5-AG), have thermal stability and exhibit excellent storage stability.

Thermostable 1,5-anhydroglucitol dehydrogenase, and method for measurement of 1,5-anhydroglucitol by using the same

A present invention relates to a method for quantitatively determining sugar-alcohols characterized by passing test samples containing sugar-alcohols, proteins, and saccharides through a column filled with basic anion-exchange resins which have a protein-removing ability and a saccharide-removing ability, and then quantitaing the sugar-alcohols in the effluent out of the column, a column filled with said resins and an aqueous solution of boric acid, and a kit. Sugar-alcohols such as 1,5-anhydroglucitol and the like have been calling attention as markers for diabetes mellitus recently.

Method for quantitatively measuring sugar-alcohol, column and kit

This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to:

Method for assaying 1,5-anhydroglucitol and kit therefor

Disclosed are an N-benzenesulfonyl derivative of cephamycin C represented by the following formula: ##STR1## which can easily be separated as a solid from an aqueous solution thereof, and a process for the preparation thereof. This derivative is valuable as an intermediate leading to a cephamycin type antibiotic substance.

Derivative of cephamycin C

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Ageo, Japan

Minoru Masuda