Sapporo Breweries Limited

Toyo Boseki Kabushiki Kaisha

Other

Daicel Chemical Industries, Ltd.

Minoru Kamimura

Masachika Takashio

Shigenori Emi

Takahide Chikano

Kazumi Yamamoto

Toshiro Kikuchi

Yoshio Kojima

Yuji Takamoto

Kohji Sugihara

Hisao Yamamoto

Tatsuro Shigyo

Shinichi Teshima

Hisao Kado

Yutaka Mitani

Toshio Kurihara

Kiichi Uchida

Suguru Takeo

Nobuhiro Watanabe

Yoshitada Mori

Tsuneo Hanyu

Toshiyuki Nishio

Yasuo Yoneda

A sugar ester derivative of the general formula (I) ##STR1## wherein G stands for a group of the formula (A) ##STR2## or a group of the formula (B) ##STR3## wherein .lambda. means 0 or 1; at least one of R.sub.1 and R.sub.2 means an ester residue of a saturated or unsaturated fatty acid having 1-30 carbon atoms and the other group means hydrogen atom or acetyl group, and R.sub.3 means a group of the formula (C) ##STR4## wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl group and its use. The sugar ester derivative of the general formula (I) is useful as a substrate for the measurement of lipase or esterase activities, and the reagents and the methods for measuring lipase or esterase activities which comprise said sugar ester derivative as the substrate are excellent in terms of reproducibility and sensitivity and enable the measurement of lipase or esterase activities in accordance with rate-assay method by an easy procedure.

Sugar ester derivatives, reagents for measurement of hydrolase activity

This invention relates to DNA containing a gene encoding uricase, a plasmid having said DNA, a transformant containing said plasmid, and a process for producing uricase by using said transformant.

DNA sequence for uricase and manufacturing process of uricase

This invention relates to an isolated uricase which is stable in an aqueous solution at a temperature up to 60.degree. C. and at a pH of 8.0 for 10 minutes.

Isolated recombinant uricase

Different from conventional uricase products, the uricase of the present invention has outstandingly high thermal stability and is active in a wide range of pH from 5 to 10 for the oxidative decomposition of uric acid undertaken in clinical analysis. The uricase of the invention is produced microbiologically by a thermophilic microorganism belonging to the genus of Bacillus and especially named as Bacillus sp. TB-90 which is a novel species distinguishable from any of the microorganisms belonging to the genus of Bacillus.

Uricase and a method for the preparation thereof

A polysaccharide RON substance having the ultraviolet absorption spectrum depicted in FIG. 1, the infrared absorption spectrum depicted in FIG. 2 and the .sup.13 C-NMR spectrum depicted in FIG. 3 is obtained from rice bran by extraction and purification; and pharmacological composition containing said RON substance.

Polysaccharide RON substance

Different from conventional urease products, the urease of the present invention has excellent stability, and has a smaller Km value. The urease of this invention is produced microbiologically by a thermophilic microorganism belonging to the genus of Bacillus and especially named as Bacillus sp. TB-90 which is a novel species distinguishable from any of the microorganisms belonging to the genus of Bacillus.

Urease and process for preparation thereof

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Yaizu, Japan

Minoru Kamimura