Toyota Jidosha Kabushiki Kaisha

Minoru Inamori

Hiroyuki Enoki

Yoshie Takeuchi

When preparing a DNA library via a nucleic acid amplification reaction using a random primer in a convenient and highly reproducible manner, amplification of DNA fragments derived from the chloroplast genome is reduced to a significant extent. A random primer comprises oligonucleotides selected from oligonucleotides group represented by TAAGAGACAGNN excluding those in which 2 bases at the 3' terminus are TG and oligonucleotides group represented by TAAGAGACAGNNN excluding those in which 3 bases at the 3′ terminus are TGC.

Set of random primers and method for preparing DNA library using the same

This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5'-CAAGCAGAAGACGGCATACGAGAT-N-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3&#x2032;, wherein Nindicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Primer for next generation sequencer and a method for producing the same, a DNA library obtained through the use of a primer for next generation sequencer and a method for producing the same, and a DNA analyzing method using a DNA library

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Kariya, Japan

Minoru Inamori