Spectral Diagnostics, Inc.

Sqi Diagnostics Systems Inc.

Genebio Systems, Inc.

Mingfu Ling

Peter Lea

Qinwei Shi

Shigui Liu

Jennifer Hansen

Methods and systems are provided for amplifying target nucleic acids by means of a polymerase with strand displacement activity, using two or more stem loop primers, each with a 3'-end portion comprising sequence complementary to a target homology site of a target nucleic acid and 5&#x2032;-end portion with a sequence comprising a stem sequence, a loop sequence and a sequence which is reverse complementary to the stem sequence, the said 5&#x2032;-end portion being capable of forming a stem loop. Such methods and systems can be used to amplify target nucleic acids isothermally by means of a polymerase with strand displacement activities.

DNA amplification via scissor-like structures (DASL)

Embodiments of the present invention relate to a three dimensional matrix of selected, synthetic peptide mimic sequences that are preferentially recognized by auto-antibodies, specifically by autoimmune antibodies, to be detected in patients afflicted with rheumatic arthritis, enabling enhanced sensitivity and specificity in detection of these antibodies in pre-symptomatic patients, in patients showing symptoms as well as patients confirmed positive for rheumatoid arthritis.

Synthetic peptides immuno-reactive with rheumatoid arthritis auto-antibodies

The present invention discloses a method for preparing a substrate coated support for use in micro-array devices. The method of the present invention comprises the steps of applying a first coat of substrate to a support, making the substrate coating ramp by subjecting the coated support to centripetal forces, adding a second coat of substrate to the resulting support having a ramping planar coat and subjecting the coated support to centripetal forces for a second time to produce a substrate coated membrane in which the thickness of the substrate layer is uniform across the entire coated surface.

Method for double-dip substrate spin optimization of coated micro array supports

Polypeptides corresponding to stable, circulating degradation products of troponin I (TnI) are described. The fragments comprise a sequence of the N-terminus of native cardiac TnI with 95-115 amino acid and additionally include fragments lacking about the 20-30 N-terminal amino acids. Utilities of these fragments and antibodies thereto include sensitive detection of myocardial infarction and purification of antibodies sensitive to the detection of troponin I degradation products.

Troponin I polypeptide fragments and uses thereof

This invention relates to single-chain polypeptides and their genetic sequences comprising a human cardiac troponin I N-terminal fragment and troponin C. The N-terminal fragment may or may not have the intact N-terminus. The single-chain polypeptide may be expressed recombinantly, and a linker or spacer polypeptide or peptide may be interposed between the troponin sequences. The single-chain polypeptides have utility as controls or calibrators for troponin assays, for the purification of troponin subunits and as an antigen for the preparation of antibodies.

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Toronto, Canada

Mingfu Ling