Boston University

Centre National De La Recherche Scientifique

L'institut Polytechnique De Bordeaux

L'université De Bordeaux

Mingfu Chen

Mark W Grinstaff

James Galagan

Uros Kuzmanovic

Catherine Klapperich

Karen Nazaretian Allen

Marjon Zamani

Margaret Chern

Chloe Grazon

Thuy Nguyen

Margarita Alexandrovna Tararina

Allison Dennis

R Baer

Described herein are methods, compositions and processes related to a microbial-based biosensor system for the detection of small molecules and analytes based on an analyte-responsive transcription factor-DNA binding mechanism with either a ratiometric fluorescent output through F&#xf6;rster resonance energy transfer (FRET) or a redox sensor output for the quantification of the target analyte with high sensitivity.

Microbial-based biosensors

Described herein is an amperometric biosensor, e.g., chronoamperometric biosensor for the measurement of the concentration of nicotine. Also disclosed herein is a wearable nicotine biosensor device and a biosensor that detects nicotine in smoke. The biosensor disclosed herein comprises a nicotine-catalyzing enzyme, such as NicA2 or mutant NicA2 enzymes. Also described herein are systems comprising said amperometric biosensor, e.g., chronoamperometric biosensor and methods of using said chronoamperometric biosensor.

Enzyme-based electrochemical nicotine biosensor

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Boston, MA, United States of America

Mingfu Chen