The Johns Hopkins University

Pioneer Hi-bred International, Inc.

Kala Pharmaceuticals, Inc.

Ming Yang

Justin Scot Hanes

Tao Yu

Elizabeth M Enlow

Alexey Popov

Hongming Chen

James Bourassa

Samuel K Lai

Laura M Ensign

Colin R Gardner

Donald Kyle

Leslie Charles Kuhlman

Andrea Beth Kalvig

Jeffrey Allan Thompson

Jin Yang

Jeffrey L Cleland

John Bryan Woodward

Joshua Michael Shendelman

Michael T McMahon

Jean Liu

Landon Linn Ries

Jie Fu

David L Hyten, Jr

Qingguo Xu

Kannie Wai Yan Chan

Ying-Ying Wang

Sarah Atwood

Hui Wang

Walter J Stark

Laura Jean Wolf

Daniel Lewis Thomas

Joshua Kays

Olcay Mert

David L Hyten

Kannie Wai Chan

Yun Yu

Michael T Mcmahon

Nanocrystals, compositions, and methods that aid particle transport in mucus are provided. In some embodiments, the compositions and methods involve making mucus-penetrating particles (MPP) without any polymeric carriers, or with minimal use of polymeric carriers. The compositions and methods may include, in some embodiments, modifying the surface coatings of particles formed of pharmaceutical agents that have a low water solubility. Such methods and compositions can be used to achieve efficient transport of particles of pharmaceutical agents though mucus barriers in the body for a wide spectrum of applications, including drug delivery, imaging, and diagnostic applications. In certain embodiments, a pharmaceutical composition including such particles is well-suited for administration routes involving the particles passing through a mucosal barrier.

Nanocrystals, compositions, and methods that aid particle transport in mucus

Various methods and compositions are provided for identifying and/or selecting a soybean plant or soybean germplasm with one or more preferred or desired reproductive growth phenotypes. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with days to initiation of flowering. In other embodiments, the method additionally comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with days to maturity. In further embodiments, the method comprises crossing a selected soybean plant with a recurrent soybean parent plant. Further provided herein are marker loci, marker alleles, primers, probes, and kits suitable for identifying and/or selecting soybean plants or soybean germplasms with one or more reproductive growth phenotypes.

Genetic loci associated with reproductive growth phenotypes in soybean and methods of use

The disclosure relates to methods and compositions for identifying and/or selecting soybean plants that have resistance to lodging, have improved resistance to lodging, or are susceptible to lodging. The methods use molecular genetic markers to identify, select and/or construct resistant plants or identify and counter-select susceptible plants. Also provided are soybean plants that display resistance or improved resistance to lodging that are generated by the methods described herein. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

QTLs associated with and methods for identifying lodging resistance in soybean

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Charcoal Rot Drought Complex. The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Soybean plants that display tolerance or improved tolerance to Charcoal Rot Drought Complex that are generated by the methods of the invention are also a feature of the invention. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Loci associated with charcoal rot drought complex tolerance in soybean

Mucus-penetrating liposomal nanoparticles and methods of making and using thereof are described herein. The nanoparticles contain one or more lipids, one or more PEG-conjugated lipids, and optionally one or more additional materials that physically and/or chemically stabilize the particles. The nanoparticle have an average diameter of about 100 nm to about 300 nm, preferably from about 100 nm to about 250 nm, more preferably from about 100 nm to about 200 nm. The particles are mobile in mucus. The liposomes can further contain one or more therapeutic, prophylactic, and/or diagnostic agent to be delivered to a mucosal surface, such as the CV tract, the colon, the nose, the lungs, and/or the eyes. The liposomes can further contain one or more CEST agents to allow real time imaging of the particles in a live animal. The particles may also further contain an imaging agent, such as a fluorescent label.

Lipid-based drug carriers for rapid penetration through mucus linings

Growing community of inventors

Towson, MD, United States of America

Ming Yang