Siemens Healthcare Diagnostics Gmbh

Caliper Life Sciences, Inc.

Ming Wu

Brian David Warner

Josh Molho

Chu-an Chang

Javier Anibal Farinas

Minxue Zheng

Stephane Mouradian

Hayley Wu

David Ahle

A microfluidic device with a filter includes a substrate; a flowpath including a well formed in the substrate in fluid communication with a channel formed in the substrate; and a filter disposed across the flowpath and associated with the channel.

Microfluidic device with a filter

The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.

Highly orthogonal universal sequences for use in nucleic acid assays

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Redwood City, CA, United States of America

Ming Wu