Agricultural Technology Research Institute

Ming-Wei Hsieh

Zeng-Weng Chen

Jiunn-Horng Lin

Jyh-Perng Wang

Chien-Yu Fang

Ping-Cheng Yang

Hsueh-Tao Liu

Weng-Zeng Huang

Tzu-Ting Peng

Shih-Ling Hsuan

Jian-Fong Lai

Hao-Zhen Zeng

Chiung-Wen Hsu

Disclosed is a composition for preventing and treating ainfection in swine. The composition uses XylF, DnaK, P72, or a combination thereof as an active pharmaceutical ingredient. Further disclosed are an expression vector and a method for producing the active pharmaceutical ingredient of the composition using a prokaryotic expression system.

Composition for preventing and treatinginfection, and method for producing said composition

The present invention provides a plasmid, method and kit for producing heat labile enterotoxin B-subunit based on aexpression system. By comparing with the conventional method in the art, the present invention has the advantages of high safety, good yield, and simplified process and is therefore favorable for the commercialization object of heat labile enterotoxin B-subunit in the application of vaccine adjuvant.

Plasmid, method and kit thereof for producing heat labile enterotoxin B-subunit

The present invention provides proteins that are suitable to be used as the active ingredient in subunit vaccine againstspp. The present invention also provides a subunit vaccine made therefrom. Said proteins have been experimentally proved to have the capability of inducing sufficient immune response to avoid pigs fromspp. infection. Said vaccine may have one of said proteins as active ingredient; or may have two or more of said proteins and is formulated as a cocktail vaccine. The present vaccine not only is safer than the conventional vaccines but also has equal or even better immune efficiency than the conventional ones. Furthermore, fusion partners suitable for producing said proteins of high solubility are also proved, which can significantly reduce production cost.

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Provided in the present invention are anti-spp. subunit vaccines, especially proteins suitable for being used as the active ingredient of thespp. subunit vaccines, and a vaccine prepared therefrom. Upon experimenting, it is confirmed that the proteins can elicit an immune response having sufficient strength to avoid the infection ofspp. in pigs. The vaccine can comprise one of the aforementioned proteins as an active ingredient, or can comprise two or more of the proteins to form a form of cocktail vaccine. The vaccine of the present invention is not only more safe than conventional vaccines, but also has equivalent or even better immune effects.

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Miaoli County, Taiwan

Ming-Wei Hsieh