Academia Sinica

National Science Council of R.o.c.

Ming-Tsair Chan

Su-May Yu

Li-Fei Liu

The present invention provides for a DNA construct comprising a promoter, a TSB1 gene, and a genetic modification, wherein the TSB1 gene and the genetic modification are promoted by the same said promoter. Additionally, the present invention provides for a plant that is transfected by this DNA construct, and a method of selecting this transfected plant.

Non-antibiotic selection marker genes

Disclosed is a method for producing a transgenic angiosperm plant, particularly a monocot, comprising the step of transforming an immature embryo of an angiosperm plant with a DNA fragment expressible in said angiosperm embryo, said DNA fragment comprising a promoter region derived from an &agr;-amylase gene of a plant, and an exogenous gene encoding a desired gene product, said promoter region being inducible under a sugar-depleted or sugar-free condition to promote the expression of said gene, whereby at least a part of the resultant transgenic angiosperm plant has the ability to express said gene product. Transgenic angiosperm plants and transgenic angiosperm plant seeds are also disclosed. The transgenic angiosperm plant seeds may have application in the industrial production of alcohol, beer, glucose and the like.

Application of &agr;-amylase gene promoter and signal sequence in the production of recombinant proteins in transgenic plants and transgenic plant seeds

The present invention is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.

Aarobacterium-mediated method for transforming rice

An expression vector comprising a promoter capable of directing expression of a coding sequence in an angiosperm cell, and a 3' untranslated region of a rice .alpha.-amylase gene, wherein, after the coding sequence is inserted into the vector, the 3' untranslated region and the coding sequence are transcribed into a single mRNA. Also featured are methods of producing a polypeptide in angiosperm cells using such a vector.

Growing community of inventors

Taipei, Taiwan

Ming-Tsair Chan