Huazhong Agricultural University

Ming Sun

Shouwen Chen

Lifang Ruan

Ziniu Yu

Jingyuan Dai

Suxia Guo

Lin Li

Ziduo Liu

Shangling Fang

Chunping Luo

Donghai Peng

Zhenyu Zhang

The isolation, cloning of an enhancin gene bel1 from, and the use of an enhancin Bel1 in biological insecticides are disclosed in the present invention. A new enhancin gene bel1 is isolated fromsubsp.YBT-1520, and its encoded product is a new enhancin Bel1. The enhancin has very strong effect-enhancing activity on the insecticidal action of insecticidal crystal protein Cry1Ac in killing Lepidoptera insects. The insecticidal activity of genetically engineeredBMB0187 developed on the basis of this enhancin is 5.7 times as high as that of the initial strain.

[object Object]

The present invention discloses the isolation, cloning and use of insecticidal crystal proteins from. The present invention isolates a novel insecticidal crystal protein gene cry7Ba1 fromsubsp.YBT-978 strain, and said gene encodes a novel insecticidal crystal protein Cry7Ba1, which shows insecticidal activity against Lepidopteran insects. The present invention also discloses the gene sequence of the novel insecticidal crystal protein, uses suitable expression vectors to transform micro-organisms so as to express the product Cry7Ba1 encoded by the gene and make it exert the insecticidal activity of the protein against Lepidopteran pests.

Growing community of inventors

Wuhan, China

Ming Sun