Nanjing Tech University

Southeast University

Ming Lei

Yong Chen

Pingkai Ouyang

Dong Liu

Hanjie Ying

Qingguo Liu

Huanqing Niu

Bin Yu

Rui Xiao

Chao Liu

Di Zhang

Weicong Xu

Shishi Wu

Yuyang Fan

Xiwei Peng

Xingyuan Cao

Yuhan Yang

Xiangchen Kong

The present invention discloses a rapid evaluation method for quality of lignin-pyrolyzed bio-oil and an application thereof, and particularly relates to a rapid evaluation method for quality of lignin-pyrolyzed bio-oil based on radical detection and an application thereof. The method can be used to evaluate the quality of lignin-pyrolyzed bio-oil by detecting the spin concentration of radicals in lignin char obtained by lignin pyrolysis, thus avoiding the complex processes involved in the evaluation for the quality of conventional pyrolyzed bio-oils such as, extraction, separation and detection and reducing the detection costs substantially. The detection method of the present invention is simple and easy to operate, thus achieving the rapid evaluation for the quality of lignin-pyrolyzed bio-oil. Moreover, the detection method of the present invention is non-contact detection without destructive samples, which is applicable to the rapid detection on the quality of lignin-pyrolyzed bio-oil in the field of industry and scientific research. The present invention further broadens the application fields of radical detection and contributes to the development of radical detection technology in the field of pyrolysis, and provides a reliable method for the detection of lignin-pyrolyzed bio-oil, which has good application prospect.

Rapid evaluation method for quality of lignin-pyrolyzed bio-oil based on radical detection and application thereof

The invention discloses a recombinantfor producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinantis constructed by overexpressing a protease gene FtsH in a; and the construction method comprises the following steps of: (1) performing PCR on a genome of the, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the, and screening to obtain the recombinant. The invention has the beneficial effects that thefor overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of theis enhanced, such that a yield of continuous immobilized fermentation of theis increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

Recombinant corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and construction method thereof

The invention discloses a method for producing lysine by utilizing adsorption and immobilized fermentation of a recombinant, wherein the recombinantis constructed by simultaneously overexpressing an adenosine triphosphate ATPase while knocking out an extracellular nuclease ExeR in a. The recombinantcan effectively improve eDNA secretion of theand reduce eDNA degradation of the, so that thecan be more easily adsorbed on a surface of a solid carrier for immobilized fermentation, such that a yield of continuous immobilized fermentation of theis increased by 49.67% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 29.17%.

Method for producing lysine by utilizing adsorption and immobilized fermentation of recombinant corynebacterium glutamicum

Growing community of inventors

Jiangsu, China

Ming Lei