National Cheng-kung University

Ming-Chi Hsieh

Dar-Bin Shieh

Tsung-Ju Li

Chih-Chia Huang

Chen-Min Chang

Po-Yang Chang

An apparatus for amplifying a nucleic acid sequence in a polymerase chain reaction (PCR) is provided. The apparatus comprises a microprocessor configured to communicatively couple with a user input device and receive instructions from the user input device and implement the received instructions; an electromagnetic radiation (EMR) generator configured to generated EMR at frequencies ranging from 300 THz to 400 THz and communicatively coupled with the microprocessor; a reaction vessel, communicatively coupled with the microprocessor, to house the nucleic acid sequence, necessary reagents to the PCR, and particles comprising a transition metal material, and to receive the EMR generated by the EMR generator; and a temperature sensor communicatively coupled with the reaction vessel and the microprocessor.

Method and device for polymerase chain reaction

Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a Polymerase Chain Reaction (PCR). The method includes contacting the reaction mixture with EMR frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a Group III metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the EMR absorbing particles with EMR having a frequency of about 200 kHz to 500 THz to amplify the target nucleic acid sequence, wherein the Group III metal is any one of Al, Ga, and In, and the transition metal is any one of Mn, Fe, Co and Cu.

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Tainan, Taiwan

Ming-Chi Hsieh