Academia Sinica

Ming-Che Shih

Wen-Hsiung Li

Jui-Jen Chang

Chieh-Chen Huang

Cheng-Yu Ho

Hao-Yeh Lin

Feng-Ju Ho

Described herein is an engineeredcell, the cell comprising in its genome: (i) two different nucleic acid molecules that each contain a promoter operably linked to a gene encoding a functional enzyme, and (ii) a selection nucleic acid molecule that contains a promoter operably linked to a gene encoding a selectable marker, wherein all of the nucleic acids molecules of (i) and (ii) are in tandem and the engineered cell expresses all of the proteins encoded by the genes of (i) and (ii).

Engineered yeast for production of enzymes

Described is a method of introducing multiple nucleic acid molecules into the genome of a cell. The method includes providing a plurality of nucleic acid molecules, each of the plurality of nucleic acid molecules containing (a) a nucleic acid sequence operatively linked to a promoter sequence at the 5' end of the nucleic acid molecule, and (b) an overlapping sequence at the 3&#x2032; end of the nucleic acid molecule.

Method for ordering and introducing multiple genes into a genome

Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.

Flavor compound-producing yeast strains

An expression system for producing a multi-enzyme complex, the system including a nucleic acid molecule containing a promoter operatively linked to a nucleotide sequence including multiple genes encoding multiple enzymes that are components of the multi-enzyme complex.

Expression system for producing multi-enzyme complexes and uses thereof

A polycistronic expression cassette encoding proteins necessary for constructing a multi-enzyme complex was developed. Also disclosed herein is a host cell containing this polycistronic expression cassette and uses thereof in degrading biomass.

Growing community of inventors

Taipei, Taiwan

Ming-Che Shih