University of California

Berkeley Lights, Inc.

Omm, Inc.

Intel Corporation

California Institute of Technology

Hrl Laboratories, LLC

Ming C Wu

Igor Y Khandros

J Tanner Nevill

Steven W Short

Aaron T Ohta

Pei-Yu E Chiou

Richard A Keller

Rhett L Affleck

Peter M Goodwin

James N Demas

Pei Yu Chiou

Gaetan L Mathieu

Anis Husain

Tom F Lue

Kevin T Chapman

Li Fan

Justin K Valley

Maurice Marcel Garcia

Kuo-Wei Huang

W Patrick Ambrose

Jeffrey T Petty

Mitchell E Johnson

Jay A Schecker

Yu-Chong Tai

James H Schaffner

John Heck

Hung P Nguyen

Tsung-Yuan Hsu

Haisheng Rong

Gregory L Tangonan

Pamela R Patterson

Adele E Schmitz

Robert Y Loo

Daniele Malleo

Matthieu Liger

Meer Nazmus Sakib

Robert N Schwartz

Tae Joon Seok

Guan-Lin Su

Sangyoon Han

Doo-Young Hah

Ji-Lin Shen

Niels Quack

Lih-Yuan Lin

Byung-Wook Yoo

Jui-che Tsai

A microfluidic apparatus can comprise a dielectrophoresis (DEP) configured section for holding a first liquid medium and selectively inducing net DEP forces in the first liquid medium. The microfluidic apparatus can also comprise an electrowetting (EW) configured section for holding a second liquid medium on an electrowetting surface and selectively changing an effective wetting property of the electrowetting surface. The DEP configured section can be utilized to select and move a micro-object in the first liquid medium. The EW configured section can be utilized to pull a droplet of the first liquid medium into the second liquid medium.

DEP force control and electrowetting control in different sections of the same microfluidic apparatus

Microfluidic devices in which electrokinetic mechanisms move droplets of a liquid or particles in a liquid are described. The devices include at least one electrode that is optically transparent and/or flexible.

Microfluidic devices with flexible optically transparent electrodes

Embodiments include apparatuses, methods, and systems including a laser device having a 1&#xd7;3 MMI coupler within a semiconductor layer. A front arm is coupled to the MMI coupler and terminated by a front reflector. In addition, a coarse tuning arm is coupled to the MMI coupler and terminated by a first back reflector for coarse wavelength tuning, a fine tuning arm is coupled to the MMI coupler and terminated by a second back reflector for fine wavelength tuning, and a SMSR and power tuning arm is coupled to the MMI coupler and terminated by a third back reflector. A gain region is above the front arm and above the semiconductor layer. Other embodiments may also be described and claimed.

Multi-mode interference (MMI) based laser devices for heterogeneous platforms

Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.

Pens for biological micro-objects

A structure for providing a boundary for a chamber in a microfluidic apparatus can comprise dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces.

Providing DEP manipulation devices and controllable electrowetting devices in the same microfluidic apparatus

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The preferred OET-DEP devices generally comprise a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a localized virtual electrode with a DEP electric field gradient of selected intensity along with input and a plurality of output fluidic channels. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles/cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

Methods and devices for sorting cells and other biological particulates

An optical switching system comprising a switching cell having first and second fixed-position bus waveguides and a moveable shunt waveguide is disclosed. The first bus waveguide includes an input and a first output. The second bus waveguide includes a second output. When the switching cell is in its unswitched state, the shunt waveguide is not optically coupled with either bus waveguide and a light signal can pass from the input to the first output while remaining in the first bus waveguide. When the switching cell is in its switched state, the shunt waveguide is optically coupled with both bus waveguides such that the light signal is coupled out of the first bus waveguide and into the second bus waveguide via the shunt waveguide. As a result, the light signal can pass from the input to the second output while bypassing the first input.

Silicon-photonics-based optical switch

A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.

Circuit-based optoelectronic tweezers

Circuit based optoelectronic tweezers

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 &#x3bc;m or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or group of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

Optoelectronic tweezers for microparticle and cell manipulation

The invention is related to methods and apparatus that manipulate droplets in a microfluidic environment. Advantageously, embodiments of the invention manipulate droplets by controlling the electro-wetting characteristics of a surface with light, thereby inducing a gradient in the surface tension of a droplet. The gradient in the surface tension propels the droplet by capillary force. A variety of operations, such as transporting, joining, cutting, and creating can be performed. Advantageously, embodiments of the invention obviate the need to create a relatively large and complex control electrode array. A plurality of photoconductive cells or a layer of a photoconductive material selectively couples an electrode carrying an electrical bias to otherwise floating conductive cells in response to a beam of light. The electrical bias applied to the conductive cell generates a localized electric field, which can change the contact angle of the droplet, thereby permitting the droplet to be propelled.

Systems and methods for optical actuation of microfluidics based on OPTO-electrowetting

An optical image-driven light induced dielectrophoresis (DEP) apparatus and method are described which provide for the manipulation of particles or cells with a diameter on the order of 100 &#x3bc;m or less. The apparatus is referred to as optoelectric tweezers (OET) and provides a number of advantages over conventional optical tweezers, in particular the ability to perform operations in parallel and over a large area without damage to living cells. The OET device generally comprises a planar liquid-filled structure having one or more portions which are photoconductive to convert incoming light to a change in the electric field pattern. The light patterns are dynamically generated to provide a number of manipulation structures that can manipulate single particles and cells or groups of particles/cells. The OET preferably includes a microscopic imaging means to provide feedback for the optical manipulation, such as detecting position and characteristics wherein the light patterns are modulated accordingly.

Growing community of inventors

Moraga, CA, United States of America

Ming C Wu