Thermo Fisher Scientific Baltics Uab

Mindaugas Ukanis

Remigijus Skirgaila

Arvydas Lubys

Renata Rimseliene

Rasa Sukackaite

Skaiste Valinskyte

Romas Tamosevicius

Karolis Matjosaitis

Ervinas Gaidamauskas

Martyna Simutyte

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3'-5&#x2032; exonuclease domain and/or a sequence non-specific dsDNA binding domain.

Thermophilic DNA polymerase mutants

A composition and method for controlled in vitro fragmentation of nucleic acids. A transposase forms catalytically active complexes with a modified transposon end that contains within its end sequence degenerate, apurinic/apyrimidinic sites, nicks, or nucleotide gaps, to fragment or shear a target nucleic acid sample in a controlled process. This method yields desired average nucleic acid fragment sizes. The inventive composition and method may be applied for generation of DNA fragments containing shortened transposon end sequences to facilitate subsequent reactions, for production of asymmetrically tailed DNA fragments, etc.

Method for controlled DNA fragmentation

Growing community of inventors

Vilnius, Lithuania

Mindaugas Ukanis