Riken Corporation

National Institute of Agrobiological Sciences

Minami Matsui

Takanari Ichikawa

Miki Nakazawa

Hirohiko Hirochika

Masaki Mori

Motoaki Seki

Kumiko Suzuki

Mika Kawashima

Yukako Hasegawa

Makoto Kobayashi

Tadao Asami

Hiroshi Takatsuji

Kazuo Shinozaki

Takeshi Nakano

Ayumi Yamagami

Shinya Takahashi

Takeshi Yoshizumi

Shoji Sugano

Nagao Hayashi

Miki Fujita

Kazuhito Gohda

Haruko Iizumi

Hirofumi Kuroda

Youichi Kondou

Kenji Oda

Akie Ishikawa

Setsuko Shimada

Chika Akagi

Tomoko Kuriyama

Hiroko Hara

Yuko Tsumoto

Yoshiharu Yamamoto

A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out onlines highly expressing rice full-length cDNA (rice-FOXlines) which were prepared by using a FOX hunting system. As a result, oneline (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.

Rice gene capable of imparting wide-spectrum disease resistance

The present invention is directed to identify genes involved in the growth of a plant and provide a transformed plant the growth of which is promoted utilizing the genes.

Transformation of a plant to promote root and/or leaf growth

It is intended to provide for a polycistronic expression pattern in a plant used as a host. The present invention provides a polynucleotide which comprises the following DNA (a) or (b) and functions as an IRES (internal ribosome entry site) in a plant: (a) DNA of the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4; or (b) DNA of a nucleotide sequence derived from the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4 by the substitution, deletion, addition, and insertion of one or more bases and having a function of positively regulating the translation of a nucleic acid located downstream thereof.

IRES functioning in plant

The present invention provides an early-maturing transgenic plant, which comprises a nucleic acid that encodes a protein having activity of causing early maturation of a plant, so that the gene can be expressed.

Early-maturing transgenic plants

This invention provides a transformed plant or alga with increased chlorophyll, comprising an overexpressed foreign DNA which codes for a chloroplast protein BPG2 or a homologue thereof, and a method for producing the transformed plant or alga.

Transformed plants or algae with highly expressed chloroplast protein BPG2

This invention is intended to identify a gene that regulates endoreduplication in a plant and to use such gene for breeding aimed at increasing the crop size. This invention provides a gene encoding an-derived protein having an amino acid sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10, or 12, which has endoreduplication promoting activity, a transgenic plant into which such gene has been introduced, thereby increasing the nuclear DNA content in the cells of such plant, and a method of using such gene to increase the size of the entire plant or a part thereof.

Gene having endoreduplication promoting activity

A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

Plant system for comprehensive gene function analysis with the use of full-length cDNA

Growing community of inventors

Kanagawa, Japan

Minami Matsui