Danisco US Inc.

E.i. Dupont De Nemours and Company

Min Qi

Quinn Qun Zhu

Perry G Caimi

Luan Tao

Paul Veikko Viitanen

Jianjun Yang

Celia Emily Gaby Payen

Paula Johanna Maria Teunissen

Perry G G Caimi

Robel Y Kahsay

Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

Yeast with improved alcohol production

Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production, particularly under high dissolved solids growth conditions. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

Yeast with improved alcohol production under high dissolved solids conditions

Described are compositions and methods relating to modified yeast that over-express polyadenylate-binding protein (PAB1). The yeast produces a deceased amount of acetate compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.

Reduction in acetate production by yeast over-expressing PAB1

The endogenous pnp gene encoding polynucleotide phosphorylase in thegenome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.

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expressing xylose isomerase fromwas found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that ofwere identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.

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Hockessin, DE, United States of America

Min Qi