Korea University Research and Business Foundation

Hyundai Motor Company

Min-Kyu Oh

Sang-Woo Lee

The present invention relates to a mutant microorganism in which a gene that encodes phosphofructokinase-2 is disrupted or deleted to reduce glycolytic flux to thereby improve the ability of the microorganism to produce N-acetylglucosamine, and to a method of producing N-acetylglucosamine using the mutant microorganism. The mutant microorganism according to the present invention has advantages in that it has high resistance to various chemical substances, grows rapidly, is easily cultured, and produces N-acetylglucosamine with high efficiency, indicating that it is useful for production of a large amount of N-acetylglucosamine.

Microorganism having improved ability to produce N-acetylglucosamine as a result of modulating glycolytic flux

The present invention relates to a method of screening a target metabolite-overproducing mutant strain using a synthetic suicide genetic circuit, and more particularly to a method of screening only a metabolite-overproducing mutant strain while killing a mutant strain that does not produce the metabolite, by introducing into mutant strains a synthetic suicide genetic circuit comprising a suicide gene coupled with a riboswitch. The method for screening a target metabolite-overproducing mutant strain according to the present invention has advantages in that a metabolite-overproducing mutant strain having a relatively fast or slow growth rate can be separated by visual observation, and in that the riboswitch that is used in the synthetic suicide genetic circuit can be replaced depending on the kind of target metabolite, and thus the synthetic suicide genetic circuit can be applied commonly to various strains.

Method for screening mutant microorganism overproducing target metabolite using synthetic suicide genetic circuit

A cylinder liner that can be accurately positioned and can securely maintain a stable state relative to a base metal of a cylinder block to thereby minimize deformation of the cylinder bore, limit abrasion of a piston and a piston ring, reduce consumption of oil and to effectively prevent damage to an engine.

Growing community of inventors

Gyeonggi-do, South Korea

Min-Kyu Oh