Fina Biosolutions, LLC

US Newwin, Inc.

Min-Ju Chang

Andrew Lees

Natalia Oganesyan

Averell L Gnatt

Tian Ye

Gaofeng Liu

The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5'-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRMprotein from. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRMas well as characterization of properly folded CRMprotein.

Production of soluble recombinant protein without n-terminal methionine

The invention is directed to the enhanced expression and purification of lipoxygenase enzymes. These enzymes are of wide-spread industrial importance, often produced in heterologous microbial systems. Preferably, the lipoxygenase produced by the methods of the invention is a plant-derived enzyme and expressed at high-levels in a microbial system that includes a protease-deficient host and one or more chaperone expression plasmids. The invention is also directed to amino acid and nucleic acid fragments of the lipoxygenase enzyme including fragments in expression constructs encoding all or a portion of one or more lipoxygenase genes. The invention is also directed to methods of manufacturing bread and other food and also non-food products with lipoxygenase manufactured by the methods of the invention.

Enhanced heterologous production of lipoxygenases

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Gaithersburg, MD, United States of America

Min-Ju Chang