The United States of America, As Represented by the Secretary, Department of Health and Human Services

Massachusetts Institute of Technology

Min Jae Song

Todd A Thorsen

Kapil Bharti

Marc Ferrer-Alegre

Johanna Bobrow

Sam Michael

Christina Zook

Russell Louis Quinn

Molly Elizabeth Boutin

Yen-Ting Tung

David Walsh

An in vitro tissue plate may include a well plate, a fluidic plate disposed on a bottom surface of the well plate, and a media manifold disposed on a bottom surface of the fluidic plate. The well plate may have at least two wells, including a tissue well and a waste well. The fluid plate may include a fluid channel extending between and fluidly connecting the tissue well to the waste well. The media manifold may include a one or more media outlets fluidly connected to the fluid channel. A tissue layer may be deposited in the tissue well. The tissue layer may include human cells such as neurovascular cells.

In vitro tissue plate

Methods are disclosed for fabricating a three-dimensional engineered blood retinal barrier (BRB) comprising a choroid and retinal pigment epithelial cells. The methods include the use of bioprinting. Also disclosed is a three-dimensional engineered BRB, and its use. Methods are also disclosed for using the three-dimensional engineered BRB, such as for the treatment of retinal degeneration in a subject or screening. A three-dimensional printing insert that is adapted for bioprinting on a culture substrate sheet that is securely retained within and exposed through a printing frame is also disclosed.

3D vascularized human ocular tissue for cell therapy and drug discovery

Growing community of inventors

Laytonsville, MD, United States of America

Min Jae Song