Inscripta, Inc.

Miles Gander

Tian Tian

Skylar Stefani

Patrick Westfall

Paul Hardenbol

Eileen Spindler

Matthew David Estes

Krishna Kannan

Phillip Belgrader

Andrew Garst

Don Masquelier

Jorge Bernate

Charles D Johnson

Bruce Chabansky

Krishna Yerramsetty

Isaac David Wagner

Thomas Hraha

Chad Douglas Sanada

Andrea Halweg-Edwards

Shea Lambert

The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

Simultaneous multiplex genome editing in yeast

Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

Methods and systems for modeling of design representation in a library of editing cassettes

The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

Increased nucleic-acid guided cell editing in yeast

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods&#x2014;including high throughput methods&#x2014;for screening cells that have been subjected to editing and identifying cells that have been properly edited.

Instruments, modules, and methods for improved detection of edited sequences in live cells

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Boulder, CO, United States of America

Miles Gander