Novartis Vaccines & Diagnostics, Gmbh

Novartis Ag

Glaxosmithkline Biologicals Sa

Mildred Ugozzoli

Derek T O'Hagan

Manmohan Singh

Gary S Ott

Mario Contorni

John J Donnelly

Jina Kazzaz

John Barackman

David Skibinski

Donatello Laera

Sandra Nuti

Derek O'hagan

John Donnelly

The invention utilizes the techniques and instruments which are typically used in flow cytometry (FC) as a tool for analyzing adsorbed materials. Thus the invention provides a method for analyzing a component which is adsorbed to an insoluble metal salt, comprising steps of: (i) labelling the adsorbed component with a binding reagent; and (ii) analyzing the labelled adsorbed component by flow cytometry.

Flow cytometry analysis of materials adsorbed to metal salts

Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(&#x3b1;-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

Microemulsions with adsorbed macromolecules and microparticles

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San Rafael, CA, United States of America

Mildred Ugozzoli