Vepex Contractor Ltd.

Mta Szegedi Biologiai Kozpontja

Reanal Finomvegyszergyar

Skandigen Ab

Miklos Kalman

Andras Simoncsits

Imre Cserpan

Laszlo Boross

Bela Szajani

Ivan Daroczi

Csaba Kari

Eva Beller

Zsuzsanna Antal Nee Magyar

Imre Suto

Gyorgy Bajszar

Bertil Aberg

A structural gene coding for authentic human serum albumin, --optionally supplemented by an upstream triplet coding for methionine and optionally extended by a synthetic prepro.sup.x -leader-coding sequence--, wherein the codons of the nucleotide sequence have been selected with regard to a non-human host, e.g. yeast, chosen for expression of authentic human serum albumin, is disclosed. Additionally there is disclosed a method of producing said gene. There are also disclosed a recombinant DNA molecule comprising said strucural gene inserted into a vector, and a host transformed with said recombinant DNA molecule. Furthermore there are disclosed a method of producing authentic human serum albumin, an authentic human serum albumin resulting from said method, and a pharmaceutical composition comprising said resulting human serum albumin.

Artificial gene coding for authentic human serum albumin, use thereof

The invention relates to a process for the preparation of oligo- and polydeoxyribonucleotides by synthesizing the complementary strand of a single-stranded DNA piece enzymatically, in the presence of deoxyribonucleoside 5'-triphosphates in a cloning vector.

Process for the preparation of oligo- and polydeoxyribonucleotides

The invention relates to a process for the immobilization of compounds comprising nucleophilic groups. According to the process of the invention a polymer comprising an amido group is swollen in a buffer. For this purpose any buffer is suitable which contains no amino, sulfhydryl and/or hydroxy groups in the molecule. To the swollen polymer a solution of a quinone - preferably p-benzoquinone - in a water miscible organic solvent is added and the mixture is activated at 273.degree.-343.degree. K. for 0.5-48 hours - preferably for 24 hours. The unreacted quinone is washed out with an aqueous solution of a water miscible organic solvent and thereafter water and/or a buffer. To the purified gel at a pH value between 3 and 11 - preferably between 6 and 8 - a buffered solution of a compound comprising a nucleophilic group is added, the suspension is incubated at 260.degree.-313.degree. K. for 24 hours, the gel is separated and the non-bound compound comprising a nucleophilic group is removed by washing.

Process for the immobilization of compounds comprising nucleophilic

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Szeged, Hungary

Miklos Kalman