Rensselaer Polytechnic Institute

Otsuka Pharmaceutical Factory, Inc.

Kirin Biomaterials Co., Ltd.

Mikiro Hayashi

Shin-ichi Hashimoto

Ryosuke Kato

Aki Nishibara

Kenta Fukunaga

Masahiro Kuratsu

The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.

Method for producing sulfated polysaccharide and method for producing PAPS

Growing community of inventors

Tokyo, Japan

Mikiro Hayashi