Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Kogyo Co., Ltd.

Mikiro Hayashi

Yoshiyuki Yonetani

Masato Ikeda

Kazuhiko Tabata

Satoshi Nakagawa

Satoshi Mitsuhashi

Hiroshi Mizoguchi

Akio Ozaki

Tetsuo Nakano

Keiko Ochiai

Shin-ichi Hashimoto

Makoto Yagasaki

Masaki Maeda

Haruhiko Yokoi

Junko Ohnishi

Seiko Ando

Akihiro Senoo

Kenji Tanaka

Yugo Adachi

Naoko Tateishi

Akihiro Senoh

Shin-Ichi Hashimoto

Provided is a novel process for producing an L-amino acid using a microorganism belonging to the genus. According to the present invention, a process for producing an L-amino acid comprising; culturing a microorganism in which an activity of the protein of any of the following [1]-[3] is increased compared with that of the parent strain in a medium, producing and accumulating the L-amino acid in the medium, and recovering the L-amino acid from the medium:

Process for production of L-amino acid

Provided is an efficient process for producing L-glutamine or L-glutamic acid using a microorganism. L-glutamine or L-glutamic acid is produced by culturing in a medium a microorganism in which has an ability to produce L-glutamine or L-glutamic acid, and in which an ability to form a superhelical double-stranded DNA is decreased compared with that of the parent strain, producing and accumulating L-glutamine or L-glutamic acid in the medium, and recovering L-glutamine or L-glutamic acid from the medium.

Process for production of L-glutamine or L-glutamic acid

The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to theoutside of the microbial cell is higher than that of a parental strain.

Method for producing dipeptide

According to the present invention, it is possible to provide a microorganism belonging to the genus, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.

Method for production of L-glutamine

A polypeptide comprising an amino acid sequence in which one or more amino acids are deleted, substituted or added in the amino acid sequence of a glutamine synthetase 2 derived from a microorganism belonging to a coryneform bacterium, wherein the coryneform bacterium producing the polypeptide has L-glutamine productivity, a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism comprising the DNA or the recombinant DNA, and a process for producing L-glutamine using the microorganism are provided.

The present invention provides a polypeptide which has: (i) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positionstofrom the N terminus of the amino acid sequence shown in SEQ ID NO: 1; or (ii) an amino acid sequence wherein one or more amino acid residues are substituted in the region at positionstofrom the N terminus of the amino acid sequence shown in SEQ ID NO: 1 and one or more amino acid residues are deleted, substituted or added in the region at positionstoorto; and which has N-acetylglutamate kinase activity.

Process for producing L-arginine, L-ornithine or L-citrulline

The present invention provides a polypeptide comprising an amino acid sequence in which at least one amino acid is deleted, substituted or added in an amino acid sequence of isopropylmalate isomerase derived from a microorganism belonging to coryneform bacteria, wherein a coryneform bacterium which produces the polypeptide comprising the amino acid sequence as the sole isopropylmalate isomerase exhibits a partial leucine requirement in a minimal medium; a DNA encoding the polypeptide, a recombinant DNA comprising the DNA, a microorganism transformed with the recombinant DNA, and a process for producing an L-amino acid using the microorganism.

Mutant isopropylmalate isomerase

Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer, and use of them.

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Ibaraki, Japan

Mikiro Hayashi