Kao Corporation

Mikio Takaiwa

Jun Hitomi

Susumu Ito

Katsuhisa Saeki

Mitsuyoshi Okuda

Tohru Kobayashi

Masafumi Nomura

Shuji Kawai

Hiromi Kubota

Shitsuw Shikata

Yasushi Kageyama

Shigehito Adachi

Yoshihiro Hakamada

Tadashi Yoshimatsu

Yoko Watanabe

Katsutoshi Ara

Eiichi Hoshino

Kazuaki Igarashi

Hiroshi Hagihara

Atsushi Tanaka

Yuichi Ohta

Masaharu Shimooka

Takaaki Uemura

An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40&#xb0; C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.

Alkaline protease

An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40&deg; C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.

Liquefying alkaline .alpha.-amylase, process for producing the same, and

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for the microorganism Bacillus sp. Ferm BP-3376, which is capable of producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline-proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Bacillus SP. ferm BP-3376 and a method for its use to produce an

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for a microorganism producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Alkaline proteinase isolated from bacillus sp.

A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism tot a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

Mutant resistant to cell membrane synthesis inhibitor and process for

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Tochigi, Japan

Mikio Takaiwa