Asahi Kogaku Kogyo Kabushiki Kaisha

Other

Kitana, Tadahiko

Nakayama, Mikio

Mikio Nakayama

Tetsuro Ogawa

Tsuneo Hiraide

Tadahiko Kitano

Ayumi Mitoh

A detection sheet capable of detecting an antigen or antibody in a biological fluid with a high sensitivity in a simple operation, as well as a detection kit and detection method using the detection sheet. The detection sheet comprises (a) a fibrous aggregate with carried particles of a calcium phosphate compound having an average particle diameter of about 0.01 to 200 microns and a Ca/P ratio of about 1.0 to 2.0, and (b) an avidin, streptavidin or avidin derivative from which carbohydrates have been extracted immobilized on said calcium phosphate compound. After a biotinylated antigen or antibody is bonded to the immobilized avidin, streptavidin, or avidin derivative of the detection sheet, the detection sheet is contacted with a test solution to thereby bond an antigen or antibody of the test solution to the detection sheet. The detection sheet is then contacted with a solution of labeling compound capable of being specifically bonded to the antigen or antibody of said test solution to detect the thus labeled antigen-antibody complex.

Sheets, kits and methods for detecting antigens or antibodies

A test plate for an agglutination test which comprises a plate with a plurality of wells formed thereon, each of the wells having adhered to a surface thereof a protein A and/or a protein G. The agglutination test plate, according to the present invention, when used in an agglutination test, can ensure a clear judgment concerning the formation of an agglutinative image or a non-agglutinative image, along with a good determination sensitivity. A process for the production of such an agglutination test plate is also provided.

Test plates for agglutination test and production process thereof

A granular polymer composite comprising polymeric granules having coated on a surface thereof a calcium phosphate compound, at least a part of the particles of the calcium phosphate compound penetrates into the polymeric granules. The composite has a high bonding strength between the polymeric granules as a matrix and a coating of the calcium phosphate compound and also can fully exhibit the functions of a calcium phosphate compound. Using the granular polymer composite, a diagnostic agent which comprises of an antigen or antibody is adsorbed and immobilized on the composite and is therefore useful in diagnostic tests utilizing an antigen-antibody reaction for a number of infectious diseases.

Granular polymer composite and production process thereof as well as

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Chiba-ken, Japan

Mikio Nakayama