Japan Agency for Marine-earth Science and Technology

Kyokuto Pharmaceutical Industrial Co., Ltd.

Mikiko Tsudome

Shigeru Deguchi

Koki Horikoshi

Susumu Ito

Kazunori Nagaki

Takuo Shiraishi

Yasunori Kurosawa

A cellulose gel medium having good visibility of microbial colonies, a cellulose gel culture substrate for manufacturing the cellulose gel medium, a method for manufacturing the cellulose gel culture substrate, a method for screening cellulase-producing microorganisms or cellulase activity with greater efficiency and rapidity, and a culture substrate, which includes a cellulose gel containing cellulose and water as medium-solidifying components, the cellulose has the viscosity of 12 to 35 mPa&#xb7;S as measured at 26&#xb0; C. with a solution prepared by dissolving the cellulose at a concentration of 2.5 mg/mL in dimethylacetamide containing 8% (W/V) lithium chloride.

Culture substrate comprising cellulose gel, solid medium using same, and cellulase activity assay method using medium

The present invention relates to a solid medium containing as a medium-solidifying component a cellulose gel, in particular a cellulose gel which is a porous cellulose gel structure containing cellulose as the skeletal part and having a cellulose concentration of 0.01% or higher and a porosity of 50% or higher, as well as a process for producing the same. The solid medium of the invention can be obtained by dispersing cellulose in a solvent, especially an aqueous thiocyanate salt solution, stirring and/or heating the dispersion to dissolve the cellulose, subsequently cooling the solution and/or removing the solvent to cause the solution to gel, and permeating nutrients into the resultant cellulose gel. The solid medium usable under a wide range of culture conditions where conventional solid media such as agar medium cannot be used, as well as a method for producing the same is provided.

Solid cellulosic culture medium and process for producing the same

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Kanagawa, Japan

Mikiko Tsudome