University of Utah Research Foundation

Arizona State University

Mikhail Skliar

Anthony Butterfield

Kevin Whitty

Mark A Hayes

Marc D Porter

Brennan Copp

Vasiliy S Chernyshev

Alexis Ramirez

Samuel Hayes

Asymmetric depth filtration for isolation of EVs or other desired nanoparticles from a biological or other fluid with high yield and purity in a simple, cost-effective manner. Such a method includes passing the biological fluid through the asymmetric depth filter (e.g., in a single pass) where the fluid is introduced into the filter at an entry portion, where components of the biological fluid pass through the wider entry portion of the pores before advancing towards the narrower exit portion of the pores, wherein EVs in the fluid become reversibly entrapped within the wider portion of the pores, while similarly sized soft, low-density lipids and/or proteins are pushed more deeply into the filter, so that the reversibly entrapped EVs can be released by simply reversing the flow, while the similarly sized soft low-density lipids and/or proteins remain permanently entrapped within the pores of the filter.

Isolation, storage, and delivery of extracellular vesicles using asymmetric depth filters

The present disclosure provides systems and methods for separating one or more analytes within a fluid mixture, and characterizing and/or detecting properties associated with the one or more analytes. In some embodiments, the systems provided herein contain a dielectrophoresis device, such as a gradient insulator-based dielectrophoresis device (g-iDEP). The present disclosure provides systems and methods for separating and characterizing analytes using particle or nanoparticle tracking analysis (NTA). NTA offers various advantages because, particle size and concentration can be calculated in real time, allowing label-free and simultaneous characterization and separation of samples with mixed and unknown analytes.

Systems and methods for non-destructive isolation, concentration, and detection for unbiased characterization of nano- and bioparticles

An ultrasonic thermometer for measuring a temperature profile along a measurement axis of a structure, comprising a continuous measurement pathway of a structure () having a longitudinal measurement axis and proximal () and distal ends (), and at least two backscatterers () formed therein transverse to the longitudinal axis and separated by a predetermined separation distance.

Ultrasonic temperature measurement device

An ultrasonic thermometer for measuring a temperature profile along a measurement axis of a solid structure, comprising a continuous measurement pathway of a solid structure () having a longitudinal measurement axis and proximal () and distal ends (), and at least two backscatterers () formed therein transverse to the longitudinal axis and separated by a predetermined separation distance.

A system and method by which thickness of a dielectric film on substrates can be noninvasively determined is invented. The system and method are especially applicable to areas and applications where traditional techniques have proven unsuccessful or limited. According to embodiments of the present invention the present system and method can be used to measure film thickness in confined and inaccessible locations, and on substrates of complex geometry. The method can be used with an arbitrary and time varying orientation of the substrate-film interface. The measurements of the film thickness on the inside of open or enclosed channels of an arbitrary geometry, and on flexible substrates are possible. With multiple embedded sensors, the film thickness in different lateral locations can be simultaneously measured. The dielectric permittivity of the FUT as a function of the distance from the substrate of the film can also be measured.

Growing community of inventors

Salt Lake City, UT, United States of America

Mikhail Skliar