Emd Millipore Corporation

Merck Patent Gmbh

Millipore Corporation

The Provost, Fellows, Foundation Scholars, and the Other Members of Board, of the College of the Holy and Undivided Trinity of Queen Elizabeth Near Dublin

Merck Millipore Ltd.

Mikhail Kozlov

Wilson Moya

Matthew T Stone

Gabriel Tkacik

William Cataldo

Herbert Lutz

Elizabeth M Goodrich

Bala Raghunath

Jonathan Steen

Joseph Parrella

Michael W Phillips

Christopher Gillespie

Nanying Bian

John Paul Amara

Philip M Goddard

Joaquin A Umana

Brian Gagnon

Senthilkumar Ramaswamy

Martin Siwak

Joseph Geringer

Joseph M Almasian

James Hamzik

Gregory Straeffer

Ajish Potty

Kevin Rautio

David Yavorsky

Jad Jaber

Sherry Ashby Leon

Jie Chen

Onur Y Kas

Stephen G Hunt

Romas Skudas

Kevin Galipeau

Jibin Hao

Jeffrey Caron

Matthias Joehnck

Dennis Aquino

David Nhiem

Ramesh Babu Padamati

Alex Xenopoulos

Bianca Edelmann

Clifton Ngan

Kamran Beyzavi

Daniel Callahan

Michael Mansfield

Murugan Rajendiran

Inga Elkina

Simon Braun

Jigneshkumar Patel

Joaquin Umana

Lars Peeck

Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

Processes for filtering liquids using single pass tangential flow filtration systems and tangential flow filtration systems with recirculation of retentate

Provided herein are electrospun or electroblown non-woven fiber membranes, methods of making such membranes and lateral flow diagnostic devices comprising such membranes.

Non-woven fiber membranes

A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

Single pass tangential flow filtration systems and tangential flow filtration systems with recirculation of retentate

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

Methods of reducing level of one or more impurities in a sample during protein purification

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions

The embodiments described herein relate to agarose ultrafiltration membrane composites and methods for making and using the same.

Agarose ultrafiltration membrane composites for size based separations

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Chromatography media and method

A composite liquid filtration platform including a composite filtration medium featuring an electrospun polymeric nanofiber layer collected on a porous membrane. When in use, the porous membrane acts as a prefilter used upstream from the polymeric nanofiber layer to remove particles from a liquid stream flowing through the composite filtration structure. The nanofiber layer, positioned downstream from the porous membrane, is used as the retentive layer for critical filtration to provide biosafety assurance, and is responsible for capturing microorganisms like bacteria, mycoplasma or viruses. The composite liquid filtration platform provided herein exhibits permeability advantages over conventional porous membranes or nanofiber mats spun on coarse non-wovens.

Nanofiber containing composite membrane structures

The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.

Purification of biological molecules

A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having aLRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

Removal of microorganisms from fluid samples using nanofiber filtration media

Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

Protein separations using an acrylamide containing filter

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal, of protein, fragments without adversely affecting the yield of the desired protein product.

Removal of fragments from a sample containing a target protein using activated carbon

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Lexington, MA, United States of America

Mikhail Kozlov