Yale University

Proteowise Inc.

Mikhail Kostylev

Erik Gunther

Stephen M Strittmatter

The present invention provides a protein separation microgel having a first plate having a first opening; a second plate; a gel substrate formed between the first plate and the second plate; and a plurality of wells positioned at a first non-zero distance from a first edge of the gel and at a second non-zero distance from a second edge of the gel; wherein the first and second edge of the gel are opposite edges of the gel.

Intragel well sample loading system

Methods of transferring bio-molecular components of individual cells in a biological sample to a solid porous substate, as well as methods of examining or detecting one or more bio-molecular components of individual cells in a biological sample. The method transferring including contacting the biological sample to a first side of the porous solid substrate having a plurality of interstices or pores extending contiguously from the first side to a second side, transferring and affixing the bio-molecular components of the biological sample to the interstices or pores of the solid substrate, and the method of examining or detecting includes detecting one or more of the bio-molecular components of the biological sample transferred to the solid porous substrate.

Biological sample capture with multiplex analysis

In one aspect, the invention provides a protein capture membrane comprising a first side and a second side and a plurality of interstices extending contiguously from the first side to the second side, wherein the interstices are coated with a protein-reactive coating; and the porous substrate comprises nanoporous alumina or porous glass. In another aspect the invention provides a method of detecting a protein of interest in a plurality of proteins.

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New Haven, CT, United States of America

Mikhail Kostylev