Katholieke Universiteit Leuven

Rijksuniversiteit Groningen

Mikhael Soskine

Giovanni Maglia

Veerle Van Meervelt

Gea Schuurman-Wolters

Annemie Biesemans

Bert Poolman

Described herein are nanopore biosensors based on a modified cytolysin protein. The nanopore biosensors accommodate macromolecules including proteins and nucleic acids, and may additionally comprise ligands with selective binding properties.

Nanopore biosensors for detection of proteins and nucleic acids

A method for detecting an analyte in a sample includes the steps of obtaining a nanopore sensor comprising a nanopore and a protein adaptor internalized in the lumen of the nanopore, adding a sample comprising an analyte to the cis side or the trans side of the nanopore, and measuring conductance across the nanopore. A change in conductance after adding the sample indicates the analyte is present in the sample and has bound to the protein adaptor. Nanopore sensors comprise a nanopore and a protein adaptor internalized in the lumen of the nanopore. The protein adaptor is a functional enzyme or ligand-binding protein.

Nanopores with internal protein adaptors

Growing community of inventors

Leuven, Belgium

Mikhael Soskine