E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Mike Lassner

James Jefferson Smith

Keith S Lowe

Derek Jantz

Dennis L Bidney

Huirong Gao

Leszek Aleksander Lyznik

Saverio Carl Falco

Andrew Mark Cigan

Carl Falco

Mark Cigan

Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.

Methods and compositions for producing male sterile plants

Growing community of inventors

Urbandale, IA, United States of America

Mike Lassner