Cytiva Sweden Ab

Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Applied Nano Surfaces Sweden Ab

Bodycote Värmebehandling Ab

Tribonex Ab

Mikael Berg

Gustav Jose Rodrigo

Bjorn Markus Olovsson

Hanno Ehring

Lars Mattsson

Enrique Napoleon Carredano

Karol Lacki

Key Hyckenberg

Roger Lundqvist

James Van Alstine

Roger Nordberg

Boris Zhmud

Gunnar Malmquist

Jamil Shanagar

Helena Skoglar

Jan-Erik Lynga

Karl Ekstrom

Henrik Sandegren

Hans Blom

Torbjorn Blank

Tomas M Karlsson

Johanna Kjorning

Mikael Carlsten

Linda Mathiasson

Catalin Andrei

Sara Sendabo

Dan Andersson

Mikael Fällström

Karl EKSTRÖM

Torbjörn Blank

Johanna KJÖRNING

Rebecka Chmielowski

Mikael FÄLLSTRÖM

Enrique Carredano

Jan-Erik LyngÅ

The present invention relates to a method for optimizing a bioprocess purification system comprising a bioreactor configured to provide a harvest comprising a target composition, and a purification process arranged downstream the bioreactor and being configured for purification of the harvest to produce a target product having a desired characteristics. The method comprising: a) detecting () at least one quality attribute indicative of characteristics of the target product in a downstream process, b) identifying () correlations between the at least one quality attribute measured in the downstream process and parameters to control a cell culture process in the bioreactor, and c) controlling () the cell culture process to meet the desired characteristics based on the identified correlations, whereby the target characteristics is within a p re-determined range.

Method in bioprocess purification system

There is provided a method of preparing a mixed liquid having a first property and a second property. The method includes providing a first set of liquids, a second set of liquids, and a third set of liquids. The method includes combining the provided sets of liquids. Further, the method includes varying at least one of the liquid flows of the first and second sets and at least one liquid flow of the third set to adjust the first property and the second property to their respective predetermined values in the resulting mixed liquid flow.

Method of preparing liquid mixtures

A method for manufacturing of steel articles comprises nitriding () a steel article at a nitrification temperature in the interval 350-650&#xb0; C., giving a nitrided steel article. The nitrided steel article is quenched () in a reactive quenching oil from the nitrification temperature. The reactive quenching oil comprises at least one of S, P, B, Mo and W. Thereby, the quenching additionally comprises coating () of the nitrided steel article by a solid lubricant comprising at least one of S, P, B, Mo and W. An apparatus for manufacturing of steel articles, a quenching oil and a steel article produced by the method are also disclosed.

Solid lubricant-coated steel articles, method and apparatus for manufacturing thereof and quenching oil used in the manufacturing

A chromatography lab system comprising a cooling compartment arranged to hold both fraction collector devices and sample containers, whereby the cooling compartment comprises an identifying device which is arranged to identify the fraction collector devices such that fractions from the chromatography are collected only in the fraction collector devices.

Chromatography lab system including cooling compartment and identifying device

There is a recognized need for novel, more simplified, approaches to isolation of plasma from whole blood, as well as a need to isolate cell-free plasma fractions containing different plasma proteins. Methods are divulged for use of aqueous phase systems, formed in blood or blood containing solutions via addition of a single polymer at relatively low concentration, to effect isolation (clarification) of plasma proteins from blood cells. Methods are also divulged to replace widely used Cohn-type plasma protein fractionation which is based on sequential addition of up to 40% (v/v) ethanol and other precipitants, with simple sequential addition of a polyacid. The latter results in isolation of plasma protein fractions (i.e. fibrinogen, immunoglobulin, albumin) in sequence similar to that obtained with Cohn Fractionation and therefore may be suitable for use to reduce solvent use and solvent-related process complications in existing plasma protein purification processes. It may also support use of polymeric film based containers in novel solvent free plasma fractionation processes. The methods disclosed may also be suitable for use in smaller scale plasma protein isolation, in research and diagnostic applications. The general methodologies are robust and can function over a broad range of process variables such as temperature and pH.

Plasma protein fractionation by sequential polyacid precipitation

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Uppsala, Sweden

Mikael Berg