Sysmex Corporation

Mika Yoshimura

Keiichiro Shohmi

Katsuhiko Jindo

Yoshinori Ooi

Shuji Yamashita

Michitaka Notoya

Yutaka Maeda

Disclosed is a nucleic acid amplification method. The method comprises: performing a first nucleic acid amplification using a target nucleic acid contained in a sample as a template; and performing a second nucleic acid amplification using the amplification product produced in the first nucleic acid amplification step as a template. In the first nucleic acid amplification, a DNA polymerase that selectively amplifies a nucleic acid not comprising a uracil base is used to produce an amplification product that does not comprise a uracil base. In the second nucleic acid amplification, (i) dUTP and/or a primer comprising a uracil base, and (ii) a DNA polymerase capable of amplifying a nucleic acid comprising a uracil base are used to produce an amplification product that comprises a uracil base.

Nucleic acid amplification method producing uracil-containing amplification products

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Kobe, Japan

Mika Yoshimura