Kao Corporation

Mika Terai

Tatsuro Ozaki

Akihito Kawahara

Yasushi Kageyama

Shenghao Liu

Provided is a method for intracellularly producing a large amount of a Cry protein in abacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming abacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a &#x3c3;A-dependent promoter or a &#x3c3;H-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.

Production method for protein

A method of producing lipids, comprising the steps of:

Method of producing lipid

A method of producing a long-chain fatty alcohol, containing culturing a microorganism wherein expression of a gene encoding a 3-ketoacyl-ACP synthase and expression of a gene encoding a fatty acyl-CoA reductase are enhanced;

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Sakai, Japan

Mika Terai