Shimadzu Corporation

Ips Portal, Inc.

Mika Okada

Takashi Inoue

Yasushi Kondo

Yoshitake Yamamoto

Shuhei Yamamoto

Yoshitaka Noda

Minoru Okada

Akari Takeda

Yamato Maeda

Yuki Kuzuhara

Potsun Chiang

A cell picking device for sucking cells from a liquid sample in a sample container includes a sucking member to which a pipette tip is attachable, a driver that moves the sucking member and performs suction through the sucking member and the pipette tip, a work mode switcher that switches a work mode of the driver between a first mode and a second mode, and a controller that controls the driver.

Cell picking device and cell picking method

A cell picking device includes a stage, a sucker, a driver, a work content receiver, a registrar and a device controller. A sample container is placed on the stage. The driver is provided to execute sample scraping work and sample sucking work using a pipette tip attached to the sucker. In a case in which selection of work contents of the driver is received by the work content receiver, a work procedure including the received work contents of the driver is registered by the registrar. The work of the driver is controlled by the device controller in accordance with the registered work procedure.

Cell picking device

A cell area extraction unit () extracts a cell area in a phase image that is created based on a hologram obtained by in-line holographic microscope (IHM). A background value acquisition unit () obtains a background value from phase values at a plurality of positions outside the cell area. An intracellular phase value acquisition unit () averages a plurality of phase values on a sampling line set at a position close to the periphery of a cell, while avoiding a central portion in which the phase value may be lowered in the cell area, to obtain an intracellular phase value. A phase change amount calculation unit () obtains the difference between the intracellular phase value and the background value. A phase change amount determination unit () compares the value of the difference with thresholds in two levels to determine whether the cell is in an undifferentiated state or an undifferentiation deviant state. It is thereby possible to automatically make a correct determination while removing the influence of a theoretical measurement error by IHM.

Cell analysis method and cell analysis system using a holographic microscope

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Kyoto, Japan

Mika Okada