Shionogi Company Limited

Hokkaido University

Mika Nakano

Yoshiaki Miura

Yasuro Shinohara

Yoko Kita

Hiroshi Yamazaki

Shinichiro Nishimura

Shin-Ichiro Nishimura

Akio Takimoto

Jun-ichi Furukawa

Michio Horiuchi

Toshiharu Kuroda

Hiroaki Motoki

To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.

Apparatus for auto-pretreating sugar chain

A means for pre-treatment in glycomic analysis of a glycoprotein is provided by the present invention. A salt of the general formula (I): wherein Z, X, R, R, M, m and n are the same as described in DESCRIPTION,is useful as a protein solubilizer, and an oligosaccharide is efficiently released from a sample if reductive alkylation and/or digestion by a proteinase are carried out under the presence of the said solubilizer at the first step in the glycomic analysis of glycoprotein derived from a living body.

Method of serum pre-treatment for glycomic analysis

Growing community of inventors

Hyogo, Japan

Mika Nakano