Ajinomoto Co., Ltd.

Mitsubishi Chemical Corporation

Mika Moriya

Kazuhiko Matsui

Hisao Ito

Yoshihiko Hara

Hiroshi Izui

Seiko Hirano

Eiji Ono

Tomoko Suzuki

Yoshihiro Usuda

Takuji Ueda

Shintaro Iwatani

Yuta Nakai

Yosuke Nishio

Yasushi Hoshino

Stephen Van Dien

Akiko Matsudaira

Keiko Noguchi

Yuichiro Tsuji

Kenzo Yokozeki

Hiroyuki Kojima

Hiroshi Matsui

Mikhail Markovich Gusyatiner

Kiyoshi Miwa

Elvira Borisovna Voroshilova

Jun Nakamura

Masakazu Sugimoto

Tatyana Viktorovna Leonova

Kenji Yamagishi

Atsushi Hayakawa

Makoto Murase

Dmitriy Vladimirovich Filippov

Miku Toyazaki

Masako Izui

Makoto Tsuchiya

Kazuyuki Hayashi

Yuri Nagai

Ryusuke Aoyama

Yuri Uehara

Yoshiko Inoue

Nobuhisa Nitta

Miki Ikuta

Akari Yokokawa

The present invention provides a composition containing linalool, wherein the composition contains a high amount of either enantiomer R-linalool or S-linalool, and has a high content rate of linalool. The present invention also provides a production method for producing the composition. The present invention further provides a composition containing volatile components including linalool, in which a content of linalool in a total content of the volatile components in the composition is 60% or more, and the linalool is present as R-linalool or S-linalool in an amount of 50% or more of the enantiomer, and a production method therefor.

Linalool composition and method of producing therefor

The present invention describes an efficient method for producing linalool. The present invention provides a method for producing linalool, the method including culturing a microorganism expressing linalool synthase in a culture medium to produce linalool. The present invention also describes a microorganism able to express linalool synthase and efficiently produce linalool.

Method of producing linalool using a microorganism

A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a C4-dicarboxylic acid-uptake carrier such as DctA, DcuA, and DcuB is increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

Method for producing L-amino acid

A method for producing of an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, which has been modified so that the acpP-fabF operon is attenuated, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

The present invention provides a method for producing L-amino acids, such as L-arginine, L-citrulline, and L-lysine, using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genusor, which has been modified to attenuate expression of one or more genes, such as the pepA, pepB, and pepD genes.

Method for producing an L-amino acid using a bacterium of the enterobacteriaceae family, having attenuated expression of gene(s) encoding peptidase

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genusorand having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

L-glutamic acid-producing bacterium and method for producing L-glutamic acid

A bacterium belonging to the genuswhich has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

Method for producing L-lysine or L-threonine

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.

Method of producing succinic acid with bacterium comprising a modified fumarate reductase gene or a modified succinate dehydrogenase gene

L-glutamic acid is produced by culturing in a medium a microorganism belonging to enterobacteria and having L-glutamic acid productivity, into which a citrate synthase gene derived from a coryneform bacterium is introduced to produce and accumulate L-glutamic acid in the medium and collecting the L-glutamic acid from the medium.

L-glutamic acid producing bacterium and process for producing L-glutamic acid

A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

A method for producing L-glutamic acid which comprises culturing a microorganism belonging to the genus Klebsiella, Erwinia or Pantoea and having an ability to produce L-glutamic acid in a culture medium, and collecting produced L-glutamic acid from the culture medium. The microbial strain used is preferably a strain which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, or a strain which increase in an activity of an enzyme catalyzing a reaction for L-glutamic acid biosynthesis.

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Enterobacter or Serratia. Further, the microorganism having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis, or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

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Kawasaki, Japan

Mika Moriya