The Ohio State University

The University of Chicago

Research Institute at Nationwide Children's Hospital

Nationwide Children's Hospital, Inc.

Mijia Lu

Jianrong Li

Mark Edward Peeples

Chuan He

Boxuan Simen Zhao

Yuanmei Ma

Prosper N Boyaka

Anzhong Li

Stefan Niewiesk

Miaoge Xue

Zijie Zhang

The current disclosure relates to methods, compositions and kits for detecting modified adenosine in a target RNA molecule. Aspects relate to a method for detecting modified adenosine in a target ribonucleic acid (RNA) comprising contacting the target RNA with an adenosine deaminase enzyme (adenosine deaminase, RNA-specific) to generate a target RNA with deaminated adenosines and sequencing the target RNA with deaminated adenosines; wherein the modified adenosine is detected when the nucleotide sequence includes adenosine within a m6A motif.

Methods and compositions for treating negative-sense single-stranded RNA virus

Embodiments disclosed herein provide compositions, methods, and uses for recombinant vectors encoding Zika virus (ZIKV) protein subunits, and immunogenic compositions thereof. Certain embodiments provide recombinant vectors encoding ZIKV nonstructural protein 1 (NS 1), and optionally, ZIKV envelope (E) protein and premembrane (prM) protein. Other embodiments provide expression cassettes comprising a promoter operably linked to a polynucleotide that encodes the ZIKV NS 1 protein, and optionally ZIKV E and prM proteins. In some embodiments, the disclosed expression cassettes can be incorporated into a vector to produce a recombinant vector. Also provided are immunogenic compositions comprising one or more recombinant vectors described herein, and methods for inducing an immune response against ZIKV in a subject comprising administering to the subject an immunologically effective dose of an immunogenic composition of the present disclosure.

Recombinant vectors encoding Zika virus protein subunits

Growing community of inventors

Columbus, OH, United States of America

Mijia Lu