Oxford Nanopore Technologies Limited

Mihaela Misca

Ruth Moysey

James White

Andrew John Heron

James Anthony Clarke

Lakmal Nishantha Jayasinghe

Elizabeth Jayne Wallace

Mark Bruce

Szabolcs Soeroes

Luke McNeill

Domenico Caprotti

Luke Mcneill

The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

SSB method

The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Enzyme construct

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Oxford, United Kingdom

Mihaela Misca