Keio University

Michiyo Okui

Nobuyoshi Shimizu

Atsushi Takayanagi

An artificial antibody library with a super-repertory (10or more) is constructed by: using a cDNA library as a template, amplifying a fragment containing the CDR1 and CDR2s regions of the VH or VL region of immunoglobulin gene and a fragment containing the CDR3 region each by the PCR method; integrating the VH library and the VL library, which are little contaminated with unexpressionable repertory and have high safety, into an non-expression vector; transferring it into a host; and then shuffling the VH region in the VH library with the VL region in the VL library.

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Tokyo, Japan

Michiyo Okui