Sysmex Corporation

Michitaka Notoya

Shuji Yamashita

Nobuhiro Kitagawa

Hideki Ishihara

Nobuyasu Hori

Kenichiro Suzuki

Tomokazu Yoshida

Yoshinobu Miura

Mika Yoshimura

Yutaka Maeda

Jun Sato

Disclosed is a liquid sending method using a sample processing chip having a flow path into which a plurality of liquids flow, and the method includes: sending, into the flow path, a first liquid held in a liquid holding portion provided in the sample processing chip by applying pressure to the liquid holding portion; sending, into the flow path, a second liquid in a storage portion provided in a liquid sending device connected to the sample processing chip, through an injection hole provided in the sample processing chip, by applying pressure to the storage portion; and forming, in the flow path, a fluid containing the first liquid having been sent from the liquid holding portion, and the second liquid having been sent through the injection hole.

Liquid sending method using sample processing chip and liquid sending device for sample processing chip

Disclosed is a nucleic acid amplification method. The method comprises: performing a first nucleic acid amplification using a target nucleic acid contained in a sample as a template; and performing a second nucleic acid amplification using the amplification product produced in the first nucleic acid amplification step as a template. In the first nucleic acid amplification, a DNA polymerase that selectively amplifies a nucleic acid not comprising a uracil base is used to produce an amplification product that does not comprise a uracil base. In the second nucleic acid amplification, (i) dUTP and/or a primer comprising a uracil base, and (ii) a DNA polymerase capable of amplifying a nucleic acid comprising a uracil base are used to produce an amplification product that comprises a uracil base.

Nucleic acid amplification method producing uracil-containing amplification products

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Kobe, Japan

Michitaka Notoya